Queen 1 . В В В В В In this functional exercise you'll be using biuret reagent. В Why is biuret reagent utilized in this useful? The biuret reagent is employed to assess the concentration of the protein since peptide provides occur together with the same rate of recurrence per amino acid in the peptide. The strength of the coloring, and therefore the absorption, is straight proportional towards the protein concentration.
Queen 2 . В В В В What is the experimental reason for constructing a regular curve? Multiple samples with known houses can be measured and graphed, which then enables the same homes to be established for the unknown trials, in this case the concentration, simply by interpolating the graph which in turn depicts the partnership between the absorbance and attentiveness.
Queen 3. В Based on the previously mentioned information (in the prac manual) as well as your own thinking, which herb do you hypothesize will have more rubisco, a single raised under the sun or 1 raised in shade? It can be hypothesised that the sun increased silverbeet flower will have even more rubisco compared to the shaded silverbeet plant.
Q four. В В В В Should the line hook up all the data points or perhaps should it be a line of best fit? В For what reason? В (2 sentences) A line of best fit since it will give an accurate representation from the relationship between absorbance and concentration between your different selections. The more samples used, the closer the results will be to the line of best fit.
Q five. В В В Is that possible to determine the protein attention of a option from the standard curve if the measured absorbance is off the scale of the y-axis, i. elizabeth. higher than the best y-value around the standard shape? В Give your reasons. В В (4 sentences) Really not possible to determine the protein attentiveness because the data gathered from your standard solutions is in a particular range (specific scale). Which means relationship between absorbance and concentration cannot be determined for anything outside of this selection.
Queen 6. В В DescribeВ twoВ ways in which you would alter the fresh...